Bacillus cell-free supernatants support canine epithelial barrier function
Bacillus cell-free supernatants support canine epithelial barrier function
- 저자: Udrea Andreea C; Larsen Katrine B; Schwarzenberg Adrian; Yde Bak Steffen; Christensen Niels; Shen Chong
- 출처: Journal of animal science, 2026
- DOI/링크: 10.1093/jas/skag164
- 분야 태그: #반려동물 #동물사료
- 정리일: 2026-07-11
TL;DR
The study supports the use of cell‑based models to explore host–microbe interactions mediated by bacterial CFS
1. 연구 배경 및 동기
- 다루는 문제: Disruption of epithelial barrier function and inflammation compromise canine health and wellbeing, particularly in the gastrointestinal tract
- 기존의 공백 / 왜 필요한가: Bacillus spp. produce bioactive components, here investigated as cell‑free supernatant (CFS), that can modulate epithelial barrier‑associated pathways
2. 관련 연구
- 기존 연구 흐름: control, respectively; P < 0.001), and BL-21 also increased IL-12A expression (1.6-fold vs
- 이 논문의 위치: Animal Feed 분야 내 기존 문헌 검토
| 구분 | 기존 연구 | 이 논문 |
|---|---|---|
| 주제 | 일반적 접근 | Bacillus 중심 |
3. 핵심 기여
- In this study, we investigated the effects of cell‑free supernatants (CFS)—the secreted products of selected Bacillus strains—using canine epithelial cell and macrophage models in vitro
- By focusing on bacterial CFS rather than live cells, the study aimed to examine mechanistic host cell responses under controlled experimental conditions
- The study supports the use of cell‑based models to explore host–microbe interactions mediated by bacterial CFS.
4. 제안 방법론
- 전체 접근 논리: control, respectively; P < 0
- 단계: In addition, BA842-CFS increased BCL2 expression and BCL2L1 abundance (1.5-fold and 2.0-fold vs. control; P < 0.05) and BL-21- and 27-CFS reduced caspase-8 expression (P < 0.05)
5. 실험 설정
| 항목 | 내용 |
|---|---|
| 대상/재료 | In conclusion, Bacillus-derived CFS modulated expression of TJ proteins, adhesion molecules, cytokines, and markers of apoptosis in |
| 처리 조건 | In addition, BA842-CFS increased BCL2 expression and BCL2L1 abundance (1.5-fold and 2.0-fold vs |
| 대조군 | - |
| 측정 방법 | Beneficial bacteria can influence host physiology through the production of bioactive compounds released during growth |
6. 실험 결과 분석
| 지표 | 변화(Δ) | 조건 · 유의성 |
|---|---|---|
| - | - | In this study, we investigated the effects of cell‑free supernatants (CFS)—the secreted products of selected Bacillus strains—using canine epithelial cell and macrophage models in vitro |
제안 기전(인과 사슬): In this study, we investigated the effects of cell‑free supernatants (CFS)—the secreted products of selected Bacillus strains—using canine epithelial cell and macrophage models in vitro. By focusing on bacterial CFS rather than live cells, the study aimed to examine mechanistic host cell responses under controlled experimental conditions. Exposure of epithelial cells to Bacillus‑derived CFS altered the expression of tight junction–associated and immune‑related genes, indicating modulation of cellular pathways involved in epithelial organization and immune signaling
7. 비판적 평가
강점
- The study supports the use of cell‑based models to explore host–microbe interactions mediated by bacterial CFS.
한계 · 의문
- 표본 크기 및 장기 추적 데이터 부재 가능성
8. 향후 연구 방향
- The study supports the use of cell‑based models to explore host–microbe interactions mediated by bacterial CFS.
9. 결론
Disruption of epithelial barrier function and inflammation compromise canine health and wellbeing, particularly in the gastrointestinal tract 연구에서 control, respectively; p < 0을 통해 in this study, we investigated the effects of cell‑free supernatants (cfs)—the secreted products of selected bacillus strains—using canine epithelial cell and macrophage models in vitro. The study supports the use of cell‑based models to explore host–microbe interactions mediated by bacterial CFS
Keywords
Bacillus, cell-free, supernatants, support, canine, epithelial